DNAX Accessory Molecule-1 Active Protein | Cd226 active protein

Recombinant Mouse DNAX Accessory Molecule-1 Protein

Cat. Num. AAA2556790

• Gene Names: Cd226; Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: DNAX Accessory Molecule-1; Recombinant Mouse DNAX Accessory Molecule-1 Protein; CD226 antigen; platelet and T cell activation antigen 1; CD226 molecule; DNAM1 adhesion glycoprotein; DNAM-1; DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; CD226; TLiSA1; Cd226 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 333

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Pro 254
• Fusion Tag: C-6His
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse DNAM1 at 2 mug/ml (100 mul/well) can bind mouse CD155 with a linear ranger of 6.4-160 ng/ml.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for Cd226 active protein

Protein Construction: Recombinant Mouse DNAX Accessory Molecule-1 is produced by our Mammalian expression system and the target gene encoding Glu19-Pro254 is expressed with a 6His tag at the C-terminus.

Background: Mouse DNAX accessory molecule-1(DNAM-1) is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. As an activating receptor, it interacts with the ligands CD155 and CD112, and activates natural killer (NK) cells via its immu-noreceptor tyrosine-based activatory motif (ITAM). Mature mouse DNAM-1 has extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a cytoplasmic region that contains motifs for binding PDZ domains. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation. Conversely, it inhibits the formation of osteoclasts. Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung.

NCBI and Uniprot Product Information

• NCBI GI #: 85540456
• NCBI GeneID: 225825
• NCBI Accession #: NP_848802.2
• NCBI GenBank Nucleotide #: NM_178687.2
• UniProt Accession #: Q8K4F0
• Molecular Weight: Molecular Mass: 27.6kDa; Actual Protein Molecular Mass: 35-50kDa
• NCBI Official Full Name: CD226 antigen isoform a
• NCBI Official Synonym Full Names: CD226 antigen
• NCBI Official Symbol: Cd226
• NCBI Official Synonym Symbols: Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
• NCBI Protein Information: CD226 antigen
• UniProt Protein Name: CD226 antigen
• Protein Family: CD226 antigen
• UniProt Gene Name: Cd226
• UniProt Synonym Gene Names: Pta1

Uniprot Description

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding.