CD226 / DNAM-1 recombinant protein

Recombinant Mouse CD226 / DNAM-1 Protein (His & Fc tag)

Cat. Num. AAA2547088

• Gene Names: Cd226; Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: CD226 / DNAM-1; Recombinant Mouse CD226 / DNAM-1 Protein (His & Fc tag); BC051526; DNAM-1; DNAM1; Pta1; TLiSA1; Cd226; CD226 / DNAM-1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 333

• Application Notes: The recombinant mouse DNAM1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 484 amino acids and has a predicted molecular mass of 55 kDa. As a result of glycosylation, rm DNAM1/Fc monomer migrates with an apparent molecular mass of 70-80 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD226 / DNAM-1 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD226, also known as PTA1 or DNAM-1, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. High rate of CD226 (Cluster of Differentiation 226) is found on the surface of natural killer cells, platelets, monocytes and a subset of T cells. CD226 have binding sites with CD112 and CD155 and mediate cellular adhesion to other cells containing its ligands.

Description: A DNA sequence encoding the mouse DNAM1 (NP_848802.2) extracellular domain (Met 1-Pro 254) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 85540456
• NCBI GeneID: 225825
• NCBI Accession #: NP_848802.2
• NCBI GenBank Nucleotide #: NM_178687.2
• UniProt Accession #: Q8K4F0
• Molecular Weight: 38,063 Da
• NCBI Official Full Name: CD226 antigen isoform a
• NCBI Official Synonym Full Names: CD226 antigen
• NCBI Official Symbol: Cd226
• NCBI Official Synonym Symbols: Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
• NCBI Protein Information: CD226 antigen
• UniProt Protein Name: CD226 antigen
• UniProt Gene Name: Cd226
• UniProt Synonym Gene Names: Pta1

Uniprot Description

DNAM-1: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18|18 E4

Cellular Component: cell surface; cell-cell adherens junction; external side of plasma membrane; integral to plasma membrane

Molecular Function: cell adhesion molecule binding; integrin binding; protein binding; protein homodimerization activity; protein kinase binding; receptor activity; receptor binding

Biological Process: cell recognition; cytokine production; heterophilic cell adhesion; homophilic cell adhesion; positive regulation of immunoglobulin mediated immune response; positive regulation of interferon-gamma production; positive regulation of mast cell activation; positive regulation of natural killer cell cytokine production; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target