CD226 active protein

CD226 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122625

• Gene Names: Cd226; Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CD226; CD226 Protein; Mouse; Recombinant (His Tag); Mouse CD226/DNAM-1 Protein (His Tag); BC051526 Protein; DNAM-1 Protein; DNAM1 Protein; Pta1 Protein; TLiSA1 Protein; CD226 molecule; CD226 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro254

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse DNAM1 at 2ug/ml (100uL/well) can bind mouse CD155 with a linear range of 6.4-160ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse DNAM1 (NP_848802.2) extracellular domain (Met 1-Pro 254) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse DNAM1 at 2 ug/ml (100 ul/well) can bind mouse CD155 with a linear ranger of 6.4-160 ng/ml.)

Related Product Information for CD226 active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD226, also known as PTA1 or DNAM-1, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. High rate of CD226 (Cluster of Differentiation 226) is found on the surface of natural killer cells, platelets, monocytes and a subset of T cells. CD226 have binding sites with CD112 and CD155 and mediate cellular adhesion to other cells containing its ligands.

Product Categories/Family for CD226 active protein

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.

NCBI and Uniprot Product Information

• NCBI GI #: 85540456
• NCBI GeneID: 225825
• NCBI Accession #: NP_848802.2
• NCBI GenBank Nucleotide #: NM_178687.2
• UniProt Accession #: Q8K4F0
• Molecular Weight: 38,063 Da
• NCBI Official Full Name: CD226 antigen isoform a
• NCBI Official Synonym Full Names: CD226 antigen
• NCBI Official Symbol: Cd226
• NCBI Official Synonym Symbols: Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
• NCBI Protein Information: CD226 antigen; platelet and T cell activation antigen 1; platelet and T-cell activation antigen 1
• UniProt Protein Name: CD226 antigen
• Protein Family: CD226 antigen
• UniProt Gene Name: Cd226
• UniProt Synonym Gene Names: Pta1
• UniProt Entry Name: CD226_MOUSE

Uniprot Description

DNAM-1: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: integrin binding; protein binding; cell adhesion molecule binding; protein kinase binding

Biological Process: positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; cytokine production; positive regulation of natural killer cell mediated cytotoxicity; cell adhesion; positive regulation of mast cell activation