Typical Testing Data/Standard Curve (for reference only)
Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit | 15-LO clia kit
Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to 15-LO. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of 15-LO. You can calculate the concentration of 15-LO in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.31; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Motility/polarity/chemotaxis; Oxidoreductase; EC 1.13.11.33
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol
Molecular Function: hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; protein binding; phosphatidylinositol-4,5-bisphosphate binding; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding
Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; lipoxygenase pathway; positive regulation of actin filament polymerization; wound healing; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; bone mineralization; apoptotic cell clearance
Research Articles on 15-LO
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Product Notes
The Human 15-LO alox15 (Catalog #AAA2532388) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532388 CLIA Kit recognizes Human 15-LO. It is sometimes possible for the material contained within the vial of "15-LO (Arachidonate 15-Lipoxygenase), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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