Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit | ALOX15 elisa kit

Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit

Cat. Num. AAA2702672

• Gene Names: ALOX15; LOG15; 12-LOX; 15-LOX; 15-LOX-1
• Reactivity: Human
• Synonyms: Arachidonate-15-Lipoxygenase (ALOX15); Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit; LOX15; 15-LOX2; 15-LOX1; 15-LOX; 12-LOX; ALOX15; 15-LO; 15-LPG; LOG15; Lipoxygenase 15; Arachidonate 12-lipoxygenase; leukocyte-type; Arachidonate omega-6 lipoxygenase; ALOX15 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arachidonate-15-Lipoxygenase (ALOX15).; No significant cross-reactivity or interference between Arachidonate-15-Lipoxygenase (ALOX15) and analogues was observed.
• Sequence Length: 662

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.58ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-15-Lipoxygenase (ALOX15) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-15-Lipoxygenase (ALOX15) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOX15 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-15-Lipoxygenase (ALOX15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-15-Lipoxygenase (ALOX15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40316937
• NCBI GeneID: 246
• NCBI Accession #: NP_001131.3
• NCBI GenBank Nucleotide #: NM_001140.4
• UniProt Accession #: P16050; Q8N6R7; Q99657; A8K2P4; B7ZA11
• Molecular Weight: 70,108 Da
• NCBI Official Full Name: arachidonate 15-lipoxygenase
• NCBI Official Synonym Full Names: arachidonate 15-lipoxygenase
• NCBI Official Symbol: ALOX15
• NCBI Official Synonym Symbols: LOG15; 12-LOX; 15-LOX; 15-LOX-1
• NCBI Protein Information: arachidonate 15-lipoxygenase
• UniProt Protein Name: Arachidonate 15-lipoxygenase
• UniProt Gene Name: ALOX15
• UniProt Synonym Gene Names: LOG15; 12-LOX

NCBI Description

This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]

Uniprot Description

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.