Typical Testing Data/Standard Curve (for reference only)
Human FSCN (Fascin) CLIA Kit | FSCN clia kit
Human FSCN (Fascin) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to FSCN. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FSCN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FSCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of FSCN. You can calculate the concentration of FSCN in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
Uniprot Description
Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 7p22
Cellular Component: actin cytoskeleton; cell projection membrane; cytoplasm; cytoskeleton; cytosol; filopodium; growth cone; intercellular junction; lamellipodium; microvillus; myelin sheath; podosome; ruffle; stress fiber
Molecular Function: actin binding; actin filament binding; drug binding; protein binding; protein binding, bridging
Biological Process: actin cytoskeleton organization and biogenesis; actin filament bundle formation; cell migration; cell motility involved in cell locomotion; cell proliferation; establishment of apical/basal cell polarity; intercellular junction assembly; microspike biogenesis; positive regulation of filopodium formation; regulation of actin cytoskeleton organization and biogenesis; regulation of microvillus biogenesis
Research Articles on FSCN
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Product Notes
The Human FSCN fscn1 (Catalog #AAA2530680) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530680 CLIA Kit recognizes Human FSCN. It is sometimes possible for the material contained within the vial of "FSCN (Fascin), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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