Western Blot (WB)
(Western blot analysis of extracts from SK-BR-3 cells untreated, treated with Insulin and EGF or treated with Insulin EGF and U0126 using Rabbit anti EIF4E (pSer209) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml for 30 minutes) and Insulin (100nM for 10 minutes) or treatment with U0126.)
Rabbit anti-Human EIF4E Polyclonal Antibody | anti-EIF4E antibody
Rabbit anti EIF4E (pSer209)
WB: 1:500-1:2000
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of extracts from SK-BR-3 cells untreated, treated with Insulin and EGF or treated with Insulin EGF and U0126 using Rabbit anti EIF4E (pSer209) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml for 30 minutes) and Insulin (100nM for 10 minutes) or treatment with U0126.)
Western Blot (WB)
(Western blot analysis of extracts from SK-BR-3 cells untreated, treated with Insulin and EGF or treated with Insulin EGF and U0126 using Rabbit anti EIF4E (pSer209) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml for 30 minutes) and Insulin (100nM for 10 minutes) or treatment with U0126.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti EIF4E (pSer209) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti EIF4E (pSer209) antibody)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Research Articles on EIF4E
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Product Notes
The EIF4E eif4e (Catalog #AAA225234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti EIF4E (pSer209) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4E can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-Paraffin: 1:50-1:100 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the EIF4E eif4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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