Rabbit anti-Human Itk Polyclonal Antibody | anti-Itk antibody

Itk (IL-2 Inducible T-cell Kinase, Emt, Kinase EMT, LYK, MGC126257, MGC126258, PSCTK2, T Cell Signaling Protein, T-cell-specific Kinase, Tsk, Tyrosine Protein Kinase ITK/TSK, Tyrosine-protein Kinase Lyk) (Azide free) (HRP)

Cat. Num. AAA6483682

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Itk; Polyclonal Antibody; Itk (IL-2 Inducible T-cell Kinase; Emt; Kinase EMT; LYK; MGC126257; MGC126258; PSCTK2; T Cell Signaling Protein; T-cell-specific Kinase; Tsk; Tyrosine Protein Kinase ITK/TSK; Tyrosine-protein Kinase Lyk) (Azide free) (HRP); EC=2.7.10.2; anti-Itk antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITK.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Itk antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 169-198 in the central region of human ITK, UniProt Accession #Q08881.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(I9200-01E is used in Western blot to detect ITK in CEM cell lysate.)

Related Product Information for anti-Itk antibody

ITK is an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

Product Categories/Family for anti-Itk antibody

Antibodies; Protein Kinases

References

1. Brazin, K.N., et al., Proc. Natl. Acad. Sci. USA 99(4):1899-1904 (2002). 2. Hawkins, J., et al., Protein Expr. Purif. 22(2):211-219 (2001). 3. J anis, E.M., et al., Genomics 23(1):269-271 (1994). 4. G ibson, S., et al., Blood 82(5):1561-1572 (1993). 5. Tanaka, N., et al., FEBS Lett. 324(1):1-5 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 585361
• NCBI GeneID: 3702
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 620
• NCBI Official Full Name: Tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2-inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; homolog of mouse T-cell itk/tsk; interleukin-2-inducible T cell kinase; interleukin-2-inducible T
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN