Human Fascin (FSCN) ELISA Kit | FSCN elisa kit

Human Fascin (FSCN) ELISA Kit

Cat. Num. AAA2021912

• Gene Names: FSCN1; HSN; SNL; p55; FAN1
• Reactivity: Human
• Synonyms: Fascin (FSCN); Human Fascin (FSCN) ELISA Kit; FSCN1; SNL; p55; Singed-like protein; 55 kDa actin-bundling protein; Fascin homolog 1; Actin-Bundling Protein(Strongylocentrotus Purpuratus); Singed; Drosophila; Homolog-Like; FSCN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fascin (FSCN).; No significant cross-reactivity or interference between Fascin (FSCN) and analogues was observed.
• Sequence Length: 493

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.131ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fascin (FSCN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fascin (FSCN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FSCN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fascin (FSCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fascin (FSCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fascin (FSCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fascin (FSCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FSCN elisa kit

Metabolic pathway; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 4507115
• NCBI GeneID: 6624
• NCBI Accession #: NP_003079.1
• NCBI GenBank Nucleotide #: NM_003088.3
• UniProt Accession #: Q16658; Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
• Molecular Weight: 54,530 Da
• NCBI Official Full Name: fascin
• NCBI Official Synonym Full Names: fascin actin-bundling protein 1
• NCBI Official Symbol: FSCN1
• NCBI Official Synonym Symbols: HSN; SNL; p55; FAN1
• NCBI Protein Information: fascin
• UniProt Protein Name: Fascin
• UniProt Gene Name: FSCN1
• UniProt Synonym Gene Names: FAN1; HSN; SNL
• UniProt Entry Name: FSCN1_HUMAN

NCBI Description

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Uniprot Description

Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm; stress fiber; cytosol; cell junction; filopodium; actin cytoskeleton

Molecular Function: actin filament binding; protein binding, bridging; protein binding; drug binding; actin binding

Biological Process: actin filament bundle formation; cell proliferation; cell migration; cell motility involved in cell locomotion; actin cytoskeleton organization and biogenesis