Human Fascin ELISA Kit | FSCN1 elisa kit

Human Fascin ELISA Kit

Cat. Num. AAA2882767

• Gene Names: FSCN1; HSN; SNL; p55; FAN1
• Reactivity: Human
• Synonyms: Fascin; Human Fascin ELISA Kit; 55 kDa actin-bundling protein; Singed-like protein; p55; FSCN1; FAN1; HSN; SNL; FSCN1 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Fascin. No significant cross-reactivity or interference was observed.
• Sequence Length: 493

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.34ng/mL
• Intra-Assay CV: <=4.9%
• Inter-Assay CV: <=8.2%
• Recovery: 101%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FSCN1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Fascin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fascin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Fascin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Fascin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Fascin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507115
• NCBI GeneID: 6624
• NCBI Accession #: NP_003079.1
• NCBI GenBank Nucleotide #: NM_003088.3
• UniProt Accession #: Q16658; Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
• Molecular Weight: 54,530 Da
• NCBI Official Full Name: fascin
• NCBI Official Synonym Full Names: fascin actin-bundling protein 1
• NCBI Official Symbol: FSCN1
• NCBI Official Synonym Symbols: HSN; SNL; p55; FAN1
• NCBI Protein Information: fascin
• UniProt Protein Name: Fascin
• Protein Family: Fascin
• UniProt Gene Name: FSCN1
• UniProt Synonym Gene Names: FAN1; HSN; SNL
• UniProt Entry Name: FSCN1_HUMAN

NCBI Description

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Uniprot Description

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.