Rabbit CSE Polyclonal Antibody | anti-CSE antibody

Gamma cystathionase Polyclonal Antibody

Cat. Num. AAA2524281

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Antigen affinity purification
• Synonyms: CSE; Polyclonal Antibody; Gamma cystathionase Polyclonal Antibody; CTH; Cystathionine gamma lyase; Gamma cystathionase; anti-CSE antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Form/Format: PBS with 0.1% sodium azide, 50% glycerol, pH 7.3

• Applicable Applications for anti-CSE antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:400; IF: 1:50-1:200
• Immunogen: Fusion protein of Gamma cystathionase
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles

Western Blot

(Mouse liver tissue were subjected to SDS-PAGE followed by western blot with Gamma cystathionase antibody at dilution of 1:1000)

Immunohistochemistry

(Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Gamma cystathionase Polyclonal Antibody at dilution of 1:200 (under 40x lens).)

Immunofluorescence

(Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells usingGamma cystathionase PolyclonalAntibody at dilution of 1:50 and AlexaFluor 488-congugated AffiniPureGoat Anti-Rabbit IgG(H+L))

Related Product Information for anti-CSE antibody

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation.It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.

NCBI and Uniprot Product Information

• UniProt Accession #: P32929
• Molecular Weight: Calculated MW: 405aa, 45 kDa; Observed MW: 43-45 kDa, 66-70 kDa
• Protein Family: Caffeoylshikimate esterase

Uniprot Description

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.