Mouse CTH Monoclonal Antibody | anti-CTH antibody

CTH Monoclonal Antibody

Cat. Num. AAA2541922

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Protein G purification
• Synonyms: CTH; Monoclonal Antibody; CTH Monoclonal Antibody; CGL; cystathionase (cystathionine gamma-lyase); Cystathionine gamma lyase; Cystathionine gamma-lyase; Cysteine desulfhydrase; Gamma cystathionase; Gamma-cystathionase; Homoserine deaminase; Homoserine dehydratase; MGC9471; anti-CTH antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 620
• Purity/Purification: Protein G purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Sequence Length: 373

• Applicable Applications for anti-CTH antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:2000-1:6000; IHC: 1:200-1:800
• Immunogen: Fusion protein of Gamma
• Conjugation: Unconjugated
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of HeLa cells using CTH Monoclonal Antibody at dilution of 1:4000.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using CTH Monoclonal Antibody at dilution of 1:400)

Related Product Information for anti-CTH antibody

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309).

Product Categories/Family for anti-CTH antibody

Cancer; Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 299473759
• NCBI GeneID: 1491
• NCBI Accession #: NP_001177392.1
• NCBI GenBank Nucleotide #: NM_001190463.1
• UniProt Accession #: P32929; Q53FB3; Q53Y79; Q9H4W7; Q9H4W8; B4E1R2; E9PDV0
• Molecular Weight: Calculated MW: 405aa,45kDa ; Observed MW: 42 kDa
• NCBI Official Full Name: cystathionine gamma-lyase isoform 3
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: CTH
• NCBI Protein Information: cystathionine gamma-lyase
• UniProt Protein Name: Cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase
• UniProt Gene Name: CTH

NCBI Description

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.