Western Blot (WB)
(Anti- Cystathionase Picoband antibody, MBS178371, Western blottingAll lanes: Anti Cystathionase (MBS178371) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 45KDObserved bind size: 45KD )
Cystathionase Polyclonal Antibody | anti-CTH antibody
Anti-Cystathionase Antibody
Predicted to work with: Human
Predicted to work with: Human
Western Blot (WB)
(Anti- Cystathionase Picoband antibody, MBS178371, Western blottingAll lanes: Anti Cystathionase (MBS178371) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 45KDObserved bind size: 45KD )
Western Blot (WB)
(Anti- Cystathionase Picoband antibody, MBS178371, Western blottingAll lanes: Anti Cystathionase (MBS178371) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 45KDObserved bind size: 45KD )
Background: Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]
Uniprot Description
CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 4.4.1.1; Amino Acid Metabolism - cysteine and methionine; Cell cycle regulation; Other Amino Acids Metabolism - selenoamino acid; Energy Metabolism - nitrogen; Amino Acid Metabolism - glycine, serine and threonine; Lyase
Chromosomal Location of Human Ortholog: 1p31.1
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: calmodulin binding; carbon-sulfur lyase activity; cystathionine gamma-lyase activity; homocysteine desulfhydrase activity; protein binding; pyridoxal phosphate binding
Biological Process: activation of NF-kappaB transcription factor; cysteine biosynthetic process; cysteine biosynthetic process via cystathionine; cysteine metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein homotetramerization; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; sulfur amino acid catabolic process; transsulfuration; unfolded protein response
Disease: Cystathioninuria
Research Articles on CTH
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Product Notes
The CTH cth (Catalog #AAA178371) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cystathionase Antibody reacts with Mouse, Rat Predicted to work with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cystathionase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the CTH cth for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cystathionase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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