Rabbit CTH Polyclonal Antibody | anti-CTH antibody

CTH Polyclonal Antibody

Cat. Num. AAA3006936

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CTH; Polyclonal Antibody; CTH Polyclonal Antibody; Cystathionine gamma-lyase; Cysteine-protein sulfhydrase; Gamma-cystathionase; anti-CTH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CTH polyclonal antibody detects endogenous levels of CTH protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 405

• Applicable Applications for anti-CTH antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human CTH
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CTH polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-CTH antibody

CTH (cystathionine gamma-lyase), also known as CSE or gamma-cystathionase, is a member of the trans-sulfuration enzyme family and participates in the trans-sulfuration pathway. CTH is a cytoplasmic enzyme produced in the cytosol and is responsible for catalyzing the pyridoxal phosphate-dependent beta-disulfide elimination reaction resulting in ammonium, pyruvate and thiocysteine. The thiocysteine that is produced may then react with other thiols (or cysteine) and form hydrogen sulfide (H2S). Thus, CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion.

NCBI and Uniprot Product Information

• NCBI GI #: 27735163
• NCBI GeneID: 1491
• UniProt Accession #: P32929
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: Cystathionine gamma-lyase
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: CTH
• NCBI Protein Information: cystathionine gamma-lyase
• UniProt Protein Name: Cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase
• UniProt Gene Name: CTH
• UniProt Entry Name: CGL_HUMAN

NCBI Description

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Energy Metabolism - nitrogen; Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - selenoamino acid; Cell cycle regulation; EC 4.4.1.1; Lyase

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; cystathionine gamma-lyase activity; carbon-sulfur lyase activity; homocysteine desulfhydrase activity; pyridoxal phosphate binding

Biological Process: cysteine metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; unfolded protein response; sulfur amino acid metabolic process; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; transsulfuration; sulfur amino acid catabolic process; cysteine biosynthetic process; activation of NF-kappaB transcription factor; protein homotetramerization

Disease: Cystathioninuria