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Immunohistochemistry (IHC) (Anti-DEPDC5 antibody IHC staining of human lung, alveoli and macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit anti-Human DEPDC5 Polyclonal Antibody | anti-DEPDC5 antibody

Anti-DEPDC5 Antibody (aa1404-1432) IHC-plus

Gene Names
DEPDC5; DEP.5; FFEVF
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Immunoaffinity purified
Synonyms
DEPDC5; Polyclonal Antibody; Anti-DEPDC5 Antibody (aa1404-1432) IHC-plus; Rabbit Polyclonal to Human DEPDC5; Human DEPDC5; DEP domain containing 5; DEP.5; KIAA0645; anti-DEPDC5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human DEPDC5
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
1603
Applicable Applications for anti-DEPDC5 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry (1:10 - 1:50)
IHC-P (5 ug/ml)
WB (1:1000)
Immunogen
DEPDC5 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human DEPD5.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human DEPD5.
Immunogen Type
Synthetic peptide
Antigen Modification
aa1404-1432
Target Species
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-DEPDC5 antibody IHC staining of human lung, alveoli and macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-DEPDC5 antibody IHC staining of human lung, alveoli and macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of DEPD5 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. DEPD5 Antibody (arrow) was detected using the purified antibody.)

Western Blot (WB) (Western blot of DEPD5 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. DEPD5 Antibody (arrow) was detected using the purified antibody.)

Flow Cytometry (FC/FACS)

(DEPD5 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (DEPD5 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-DEPDC5 antibody
DEPDC5 Antibody, DEP domain containing 5 Antibody, DEP.5 Antibody, KIAA0645 Antibody Description: DEPDC5 is a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
177,910 Da
NCBI Official Full Name
DEP domain-containing protein 5
NCBI Official Synonym Full Names
DEP domain containing 5
NCBI Official Symbol
DEPDC5
NCBI Official Synonym Symbols
DEP.5; FFEVF
NCBI Protein Information
DEP domain-containing protein 5
UniProt Protein Name
DEP domain-containing protein 5
UniProt Gene Name
DEPDC5
UniProt Synonym Gene Names
KIAA0645
UniProt Entry Name
DEPD5_HUMAN

NCBI Description

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytosol; lysosomal membrane; lysosome; perinuclear region of cytoplasm

Molecular Function: GTPase activator activity; protein complex binding

Biological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci

Research Articles on DEPDC5

Similar Products

Product Notes

The DEPDC5 depdc5 (Catalog #AAA249772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DEPDC5 Antibody (aa1404-1432) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DEPDC5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:10 - 1:50) IHC-P (5 ug/ml) WB (1:1000). Researchers should empirically determine the suitability of the DEPDC5 depdc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEPDC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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