Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DEP domain-containing protein 5 (DEPDC5) Standard Curve.)
Human DEP Domain-Containing Protein 5 (DEPDC5) ELISA Kit | DEPDC5 elisa kit
Human DEP Domain-Containing Protein 5 (DEPDC5) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DEP domain-containing protein 5 (DEPDC5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DEP domain-containing protein 5 (DEPDC5) Standard Curve.)
Background: DEPDC5 encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by DEPDC5 is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in DEPDC5 are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of DEPDC5 has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Uniprot Description
DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 22q12.3
Cellular Component: cytosol; lysosomal membrane; lysosome; perinuclear region of cytoplasm
Molecular Function: GTPase activator activity; protein complex binding
Biological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway; positive regulation of GTPase activity
Disease: Epilepsy, Familial Focal, With Variable Foci
Research Articles on DEPDC5
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Product Notes
The Human DEPDC5 depdc5 (Catalog #AAA9715757) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715757 ELISA Kit recognizes Human DEPDC5. It is sometimes possible for the material contained within the vial of "DEP Domain-Containing Protein 5 (DEPDC5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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