Testing Data #1
(Staining of pig peripheral blood monocytes with Mouse anti Pig Macrophages (MBS224390) followed by Goat anti Mouse IgG:FITC (MBS235149))
Mouse MACROPHAGES Monoclonal Antibody
MOUSE ANTI PIG MACROPHAGES
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
MACROPHAGES; Monoclonal Antibody; MOUSE ANTI PIG MACROPHAGES; anti-MACROPHAGES antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
BA4D5
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-MACROPHAGES antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Western Blot (WB)*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Western Blotting: Application Note: BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Western Blotting: Application Note: BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Porcine alveolar macrophages.
Fusion Partners
Fusion Partners
Target Species
Pig
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Staining of pig peripheral blood monocytes with Mouse anti Pig Macrophages (MBS224390) followed by Goat anti Mouse IgG:FITC (MBS235149))
Testing Data #1
(Staining of pig peripheral blood monocytes with Mouse anti Pig Macrophages (MBS224390) followed by Goat anti Mouse IgG:FITC (MBS235149))
Related Product Information for anti-MACROPHAGES antibody
Mouse anti Pig Macrophages antibody, clone BA4D5 recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells. It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
Similar Products
Product Notes
The MACROPHAGES (Catalog #AAA224390) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MACROPHAGES can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Western Blot (WB)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Western Blotting: Application Note: BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions. Researchers should empirically determine the suitability of the MACROPHAGES for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MACROPHAGES, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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