Testing Data
(Sheep alveolar macrophages stained with Mouse anti Sheep CD32-Like followed by Goat anti Mouse IgG:FITC (MBS235142))
Mouse CD32-Like Monoclonal Antibody | anti-CD32 antibody
MOUSE ANTI SHEEP CD32-Like
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD32-Like; Monoclonal Antibody; MOUSE ANTI SHEEP CD32-Like; anti-CD32 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
VPM63
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD32 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Sheep
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch
Testing Data
(Sheep alveolar macrophages stained with Mouse anti Sheep CD32-Like followed by Goat anti Mouse IgG:FITC (MBS235142))
Testing Data
(Sheep alveolar macrophages stained with Mouse anti Sheep CD32-Like followed by Goat anti Mouse IgG:FITC (MBS235142))
Related Product Information for anti-CD32 antibody
Mouse anti Sheep CD32-Like, clone VPM63 recognises a cell surface antigen of 40-42kDa which is similar to the human cell surface antigen designated as CD32. It has therefore been hypothesized that clone VPM63 may recognise a minor isoform of the ovine homologue of human CD32 (Hopkins, J. et. al. 1996). CD32 is one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. CD32, also known as the Fc gamma RII, acts as a low affinity receptor for IgG. The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. Clone VPM63 has been shown to recognise an antigen expressed by macrophages and monocytes but not by lymphocytes and shows strong staining of alveolar macrophages in flow cytometry.In addition to clone VPM63, clone CCG36, an antibody directed against bovine CD32 but cross reacting with ovine CD32 is also available from MyBioSource. Clone CCG36 is available in both unconjugated and conjugated formats.
Similar Products
Product Notes
The CD32 (Catalog #AAA219902) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD32-Like can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32-Like, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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