Testing Data
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16:RPE)
CD16 Monoclonal Antibody | anti-CD16 antibody
MOUSE ANTI PIG CD16:RPE
Gene Names
FCGR3B; FCGR3
Applications
Flow Cytometry
Synonyms
CD16; Monoclonal Antibody; MOUSE ANTI PIG CD16:RPE; anti-CD16 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
G7
Specificity
CD16
Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or thelow affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).
Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NKcells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicatedthat G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or thelow affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).
Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NKcells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicatedthat G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
Form/Format
RPE; Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Applicable Applications for anti-CD16 antibody
Flow Cytometry (FC)
Application Notes
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Target Species
Pig
Reconstitution
Reconstitute with 1.0 ml distilled water
Max Ex/Em
Fluorophore: RPE 488nm laser; Excitation Max (nm): 496; Emission Max (nm): 578
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Porcine peripheral blood leucocytes
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653myeloma cell line
Preparation and Storage
Store at 4°C
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
Testing Data
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16:RPE)
Testing Data
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16:RPE)
Testing Data
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16 (MBS212426GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Testing Data
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16 (MBS212426GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Related Product Information for anti-CD16 antibody
Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998). Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
FCGR3
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III; FcgammaRIII a.1; cytolytic trigger molecule G7; fc-gamma RIII; fcRIII; igG Fc receptor III
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III
Protein Family
UniProt Gene Name
FCGR3
UniProt Synonym Gene Names
IgG Fc receptor III; FcRIII
UniProt Entry Name
FCGR3_PIG
Uniprot Description
Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor.
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Product Notes
The CD16 fcgr3 (Catalog #AAA215422) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD16 fcgr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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