Mouse anti-Pig CD27 Monoclonal Antibody | anti-CD27 antibody

MOUSE ANTI PIG CD27: APC

Cat. Num. AAA224787

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Reactivity: Pig
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD27; Monoclonal Antibody; MOUSE ANTI PIG CD27: APC; anti-CD27 antibody

• Host: Mouse
• Reactivity: Pig
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: B30C7
• Specificity: CD27
• Form/Format: Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
• Sequence Length: 260

• Applicable Applications for anti-CD27 antibody: Flow Cytometry (FC/FACS)
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Preservative Stabilisers: 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin, 5% Sucrose
• Buffer Solution: Phosphate buffered saline
• Reconstitution: Reconstitute with 1.0 ml distilled water
• Preparation and Storage: Prior to reconstitution store at +4 degree C. After reconstitution store at +4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 12 months from date of reconstitution

Testing Data

(Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27 detected with Goat anti Mouse IgG:FITC , and Mouse anti Pig wCD8a:RPE )

Testing Data

(Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:RPE and Mouse anti Pig wCD8a:FITC )

Flow Cytometry (FC/FACS)

(Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:APC and Mouse anti Pig wCD8a:RPE )

Flow Cytometry (FC/FACS)

(Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:FITC and Mouse anti Pig wCD8a:RPE )

Related Product Information for anti-CD27 antibody

Mouse anti Pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however studies undertaken using the B30C7 clone have been unable to detect expression of CD27 on porcine B-cells (Reutner et al. 2012). Studies in humans have demonstrated CD27 to be involved in apoptosis, research in swine has shown that porcine CD27 is expressed by all naive CD8a-ve T-helper cells and a sub-population of CD8a+ve cells (Reutner et al. 2012).Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and research utilizing clone B30C7 has shown that this monoclonal antibody may be used to differentiate between subsets of pig NK cells (Mair et al. 2013).

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen
• UniProt Protein Name: CD27 antigen
• Protein Family: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7
• UniProt Entry Name: CD27_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Membrane protein, integral; Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: external side of plasma membrane; extracellular region; integral to plasma membrane; neuron projection; nucleus; plasma membrane

Molecular Function: caspase inhibitor activity; protein binding; transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: brain development; cell surface receptor linked signal transduction; immune response; immunoglobulin mediated immune response; induction of apoptosis via death domain receptors; inflammatory response; negative regulation of apoptosis; negative regulation of caspase activity; positive regulation of B cell differentiation; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; regulation of cell proliferation; release of cytoplasmic sequestered NF-kappaB; response to ethanol; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway

Disease: Lymphoproliferative Syndrome 2