Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC (MBS210764))
Mouse CD4a Monoclonal Antibody | anti-CD4a antibody
MOUSE ANTI PIG CD4a:RPE
Applications
Flow Cytometry, Functional Assay
Synonyms
CD4a; Monoclonal Antibody; MOUSE ANTI PIG CD4a:RPE; anti-CD4a antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
MIL17
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Applicable Applications for anti-CD4a antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers
Perservative Stabilisers
Buffer Solution
Phosphate buffered saline
Target Species
Pig
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC (MBS210764))
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC (MBS210764))
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a (MBS214043GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a (MBS214043GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Related Product Information for anti-CD4a antibody
Mouse anti Porcine CD4a, clone MIL17 recognizes a 55kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4a cluster at the ˜Third International Workshop on Swine Leukocyte Differentiation Antigens' (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily. Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987) Mouse anti Pig CD4a, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010).
Similar Products
Product Notes
The CD4a (Catalog #AAA213660) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4a can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
