Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PDPN-HA tag Transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EPO-HA tag Transfected 293T cell lysate The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human FABP2-HA tag The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human MIF-HA tag`)
Mouse anti-Human HA tag Monoclonal Antibody
HA tag antibody
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
HA tag; Monoclonal Antibody; HA tag antibody; anti-HA tag antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT5E7
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-HA tag antibody
ELISA (EIA), Western Blot (WB)
Antigen Species
Human
Immunogen
Synthetic peptide (YPYDVPDYA)-KLH
Clone Note
Anti HA tag mAb, clone AT5E7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a synthetic peptide.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PDPN-HA tag Transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EPO-HA tag Transfected 293T cell lysate The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human FABP2-HA tag The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human MIF-HA tag`)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PDPN-HA tag Transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EPO-HA tag Transfected 293T cell lysate The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human FABP2-HA tag The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human MIF-HA tag`)
Related Product Information for anti-HA tag antibody
HA tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a HA-tag allows one to follow the protein with an antibody against the HA epitope.
Product Categories/Family for anti-HA tag antibody
References
Field. J, et al (1988) Mol. Cell. Biol. 8: 2159-2165.
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Product Notes
The HA tag (Catalog #AAA206615) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HA tag antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HA tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the HA tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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