Western Blot (WB)
(Recombinant protein MBP-tagged BCDEF, TPD52 and MAGEA3 (each 20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-E Coli MBP Monoclonal Antibody | anti-MBP antibody
MBP antibody
Reactivity
E Coli
Applications
ELISA, Western Blot
Purity
By protein-G affinity chromatography
Synonyms
MBP; Monoclonal Antibody; MBP antibody; Maltose binding protein; anti-MBP antibody
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
10C12
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
160
Applicable Applications for anti-MBP antibody
ELISA (EIA), Western Blot (WB) (Recombinant protein)
Application Notes
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.
Recommended starting dilution is 1:1,000.
Antigen Species
E Coli
Immunogen
Recombinant MBP purified from E. coli
Clone Note
Anti-MBP mAb, clone 10C12, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant MBP protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Recombinant protein MBP-tagged BCDEF, TPD52 and MAGEA3 (each 20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Recombinant protein MBP-tagged BCDEF, TPD52 and MAGEA3 (each 20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-MBP antibody
Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.
Product Categories/Family for anti-MBP antibody
References
Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215; Weiming Y et al., (2001) EMBO J 20 (3):362-371; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29):10200-10205
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The MBP (Catalog #AAA200053) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MBP antibody reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's MBP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Recombinant protein). WB: 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the MBP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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