Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHODL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: HaCaT cell lysate Lane 3.: HepG2 cell lysate Lane 4.: HeLa cell lysate)
Mouse anti-Human CHODL Monoclonal Antibody | anti-CHODL antibody
CHODL antibody
Gene Names
CHODL; MT75; PRED12; C21orf68
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
CHODL; Monoclonal Antibody; CHODL antibody; Chondrolectin isoform a; C21orf68; MT75; PRED12; anti-CHODL antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT25G6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
273
Applicable Applications for anti-CHODL antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000.
Antigen Species
Human
Immunogen
Recombinant human CHODL (22-216aa) purified from E. coli
Clone Note
Anti-human CHODL mAb, clone AT25G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CHODL protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHODL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: HaCaT cell lysate Lane 3.: HepG2 cell lysate Lane 4.: HeLa cell lysate)
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHODL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: HaCaT cell lysate Lane 3.: HepG2 cell lysate Lane 4.: HeLa cell lysate)
Related Product Information for anti-CHODL antibody
Chondrolectin is an N-glycosylated, single pass type I membrane protein that localizes to the endoplasmic reticulum (ER)-Golgi apparatus. Chondrolectin contains one carbohydrate recognition (CRD) domain and is predominantly expressed in vascular muscle of testis, red pulp of spleen and smooth muscle of prostate. Chondrolectin is also found in heart muscle, skeletal muscle, and small intestine. At least two other isoforms of Chondrolectin exist due to alternative splicing.
Product Categories/Family for anti-CHODL antibody
References
Claessens A., et al. (2007) Cell Biol Int. 31 (11): 1323-1330.; Weng L., et al. (2002) Genomics. 80 (1): 62-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,418 Da
NCBI Official Full Name
chondrolectin isoform a
NCBI Official Synonym Full Names
chondrolectin
NCBI Official Symbol
CHODL
NCBI Official Synonym Symbols
MT75; PRED12; C21orf68
NCBI Protein Information
chondrolectin
UniProt Protein Name
Chondrolectin
Protein Family
UniProt Gene Name
CHODL
UniProt Synonym Gene Names
C21orf68
NCBI Description
This gene encodes a type I membrane protein with a carbohydrate recognition domain characteristic of C-type lectins in its extracellular portion. In other proteins, this domain is involved in endocytosis of glycoproteins and exogenous sugar-bearing pathogens. This protein localizes predominantly to the perinuclear region. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
May play a role in the development of the nervous system such as in neurite outgrowth and elongation. May be involved in motor axon growth and guidance.
Research Articles on CHODL
Similar Products
Product Notes
The CHODL chodl (Catalog #AAA200384) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CHODL antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHODL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:1000. Researchers should empirically determine the suitability of the CHODL chodl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHODL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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