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Western Blot (WB) (The recombinant human SNCA-Myc tag protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000) Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000) Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000) The recombinant protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human SNCA-Myc tag (20ng) Lane 2. : Recombinant Human Myc-GST tag (50ng) Lane 3. : Recombinant Human s100A8-Myc tag (50ng) Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng))

Mouse anti-Human Myc tag Monoclonal Antibody | anti-MYC antibody

Myc tag antibody

Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
Myc tag; Monoclonal Antibody; Myc tag antibody; anti-MYC antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT19B4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
454
Applicable Applications for anti-MYC antibody
ELISA (EIA), Western Blot (WB)
Antigen Species
Human
Immunogen
Recombinant human C-Myc (410-419aa) with tag purified from E. coli
Clone Note
Anti-human Myc tag mAb, clone AT19B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNCA-Myc tag protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The recombinant human SNCA-Myc tag protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000) Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000) Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000) The recombinant protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human SNCA-Myc tag (20ng) Lane 2. : Recombinant Human Myc-GST tag (50ng) Lane 3. : Recombinant Human s100A8-Myc tag (50ng) Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng))

Western Blot (WB) (The recombinant human SNCA-Myc tag protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000) Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000) Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000) The recombinant protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human SNCA-Myc tag (20ng) Lane 2. : Recombinant Human Myc-GST tag (50ng) Lane 3. : Recombinant Human s100A8-Myc tag (50ng) Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng))
Related Product Information for anti-MYC antibody
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope.
Product Categories/Family for anti-MYC antibody
References
Cotterman R., et al. (2008) Cancer Res. 68 (23): 9654-9662.; Finver SN., et al. (1988) P NATL ACAD SCI. USA 85 (9): 3052-3056.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,565 Da
NCBI Official Full Name
myc proto-oncogene protein isoform 1
NCBI Official Synonym Full Names
MYC proto-oncogene, bHLH transcription factor
NCBI Official Symbol
MYC
NCBI Official Synonym Symbols
MRTL; MYCC; c-Myc; bHLHe39
NCBI Protein Information
myc proto-oncogene protein
UniProt Protein Name
Myc proto-oncogene protein
Protein Family
UniProt Gene Name
MYC
UniProt Synonym Gene Names
BHLHE39; bHLHe39

NCBI Description

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Uniprot Description

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000).

Research Articles on MYC

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Product Notes

The MYC myc (Catalog #AAA200394) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myc tag antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myc tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the MYC myc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myc tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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