Mouse anti-Human DYKDDDDK tag Monoclonal Antibody

DYKDDDDK tag

Cat. Num. AAA206620

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: By protein-A affinity chromatography
• Synonyms: DYKDDDDK tag; Monoclonal Antibody; anti-DYKDDDDK tag antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: AT3G6
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-DYKDDDDK tag antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Immunogen: Synthetic peptide(DYKDDDDKC)-KLH
• Clone Note: Anti DYKDDDDK tag mAb, clone AT3G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a synthetic peptide.
• Preparation and Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The recombinant proteins (200ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYKDDDDK tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human 6XHis-SNCA-3xFlagThe cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYKDDDDK tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysate Lane 2.: ODC1-DYKDDDDK tag Transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYKDDDDK tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HDAC2-DYKDDDDK tag Transfected 293T cell lysate)

Related Product Information for anti-DYKDDDDK tag antibody

DYKDDDDK-tag, or DYKDDDDK octapeptide, or DYKDDDDK epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins with in living cells. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A DYKDDDDK-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a DYKDDDDK-tag to a protein allows the protein to be studied with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

References

Dave JM, et al (2016), J Cell Sci 129:743-56. Guo H, et al (2016), Genome Biol 17:24.