SDS-Page
PPM1G recombinant protein
PPM1G, 317-546aa, Human, His tag, E Coli
Gene Names
PPM1G; PP2CG; PPP2CG; PP2CGAMMA
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
PPM1G; 317-546aa; Human; His tag; E Coli; Protein phosphatase 1G; PP2CG; PPP2CG; MGC1675; MGC2870; PP2Cgamma; PP2C; PP2C gamma; Protein phosphatase 1C; Protein phosphatase 2C gamma isoform; Protein phosphatase 2 catalytic subunit gamma isoform; Protein phosphatase magnesium dependent 1 gamma.; PPM1G recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 25mM Tris pH 7.5, 1mM DTT, 1mM EDTA, 2mM beta-mercaptoethanol, 20% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD
Sequence Length
546
Applicable Applications for PPM1G recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PPM1G recombinant protein
PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Recombinant human PPM1G, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for PPM1G recombinant protein
References
Marc Flajolet., et al. (2004). Proc. Natl. Acad. Sci. 100 (26):16006-11.; Suh EJ., et al. (2007). FEBS Lett. 580 (26):6100-4.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27 kDa (250 aa), confirmed by MALDI-TOF.
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1G
NCBI Official Symbol
PPM1G
NCBI Official Synonym Symbols
PP2CG; PPP2CG; PP2CGAMMA
NCBI Protein Information
protein phosphatase 1G
UniProt Protein Name
Protein phosphatase 1G
Protein Family
UniProt Gene Name
PPM1G
UniProt Synonym Gene Names
PPM1C; PP2C-gamma
NCBI Description
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
Research Articles on PPM1G
Similar Products
Product Notes
The PPM1G ppm1g (Catalog #AAA203146) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PPM1G can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PPM1G ppm1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD. It is sometimes possible for the material contained within the vial of "PPM1G, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
