PDE4B recombinant protein

PDE4B Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120841

• Gene Names: PDE4B; DPDE4; PDE4B5; PDEIVB
• Purity: >80% as determined by SDS-PAGE
• Synonyms: PDE4B; PDE4B Protein; Human; Recombinant (His & GST Tag); Human PDE4B/DPDE4 Protein (His & GST Tag); DPDE4 Protein; PDEIVB Protein; phosphodiesterase 4B; cAMP-specific; PDE4B recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 10% gly, 0.5mM PMSF, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of
• Sequence: Met1-Thr564

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human PDE4B isofrom 2 (NP_001032416.1)(Met 1-Thr 564) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDE4B recombinant protein

Background: cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B/DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B/DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents

References

Bolger G.et al., 1993, Mol. Cell. Biol. 13:6558-71. Card G.L.et al., 2004, Structure 12:2233-47. Card G.L.et al., 2005, Nat. Biotechnol. 23:201-7. Wang H.et al., 2007, Biochem. J. 408:193-201. Hamblin J.N. et al., 2008, Bioorg. Med. Chem. Lett. 18: 4237-41.

NCBI and Uniprot Product Information

• NCBI GI #: 82799482
• NCBI GeneID: 5142
• NCBI Accession #: NP_001032416.1
• NCBI GenBank Nucleotide #: NM_001037339.1
• UniProt Accession #: Q07343; O15443; Q13945; Q5TEK4; Q5TEK5; Q5TEK6
• Molecular Weight: 83,343 Da
• NCBI Official Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform 2
• NCBI Official Synonym Full Names: phosphodiesterase 4B, cAMP-specific
• NCBI Official Symbol: PDE4B
• NCBI Official Synonym Symbols: DPDE4; PDE4B5; PDEIVB
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4B; PDE32; dunce-like phosphodiesterase E4; cAMP-specific phosphodiesterase-4 B isoform; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE4B
• UniProt Synonym Gene Names: DPDE4
• UniProt Entry Name: PDE4B_HUMAN

Uniprot Description

PDE4B: a cAMP-specific phosphodiesterase. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. Inhibited by rolipram. Three alternatively-spliced isoforms have been described.

Protein type: Phosphodiesterase; EC 3.1.4.53; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: perinuclear region of cytoplasm; voltage-gated calcium channel complex; cytosol; nucleus; Z disc

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; metal ion binding; cAMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: neutrophil chemotaxis; positive regulation of interferon-gamma production; cAMP catabolic process; positive regulation of interleukin-2 production; neutrophil homeostasis; T cell receptor signaling pathway; leukocyte migration