PPM1G active protein

Human Recombinant PPM1G

Cat. Num. AAA844335

• Gene Names: PPM1G; PP2CG; PPP2CG; PP2CGAMMA
• Applications: SDS-Page
• Purity: >=85%
• Synonyms: PPM1G; Human Recombinant PPM1G; Protein phosphatase 1G; PP2CG; PPP2CG; MGC1675; MGC2870; PP2CGAMMA.; PPM1G active protein

• Host: E Coli
• Purity/Purification: >=85%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT, 0.1 M NaCl, and 0.1 mM PMSF.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
• Sequence Length: 546

• Applicable Applications for PPM1G active protein: SDS-PAGE
• Biological Activity: Specific activity: >5,000 units/mg.
• Results: >5,000 units/mg
• Unit Definition: One unit will hydrolyze 1 nmole of p-nitrophenylphosphatate (pNPP) per minute at 37 degree C, pH 7.5 using 10 mM of substrate.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PPM1G active protein

Background: PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.

Product Categories/Family for PPM1G active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 29826282
• NCBI GeneID: 5496
• NCBI Accession #: NP_817092.1
• NCBI GenBank Nucleotide #: NM_177983.2
• UniProt Accession #: O15355
• Molecular Weight: 61.4 kDa (566 aa, 1-546 aa + NT His-Tag)
• NCBI Official Full Name: protein phosphatase 1G
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1G
• NCBI Official Symbol: PPM1G
• NCBI Official Synonym Symbols: PP2CG; PPP2CG; PP2CGAMMA
• NCBI Protein Information: protein phosphatase 1G
• UniProt Protein Name: Protein phosphatase 1G
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1G
• UniProt Synonym Gene Names: PPM1C; PP2C-gamma
• UniProt Entry Name: PPM1G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Uniprot Description

PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Spliceosome; EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; cell cycle arrest