Western Blot (WB)
(The 293T cell lysate (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human PPM1G Monoclonal Antibody | anti-PPM1G antibody
PPM1G antibody
Gene Names
PPM1G; PP2CG; PPP2CG; PP2CGAMMA
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
By protein-G affinity chromatography
Synonyms
PPM1G; Monoclonal Antibody; PPM1G antibody; Protein phosphatase 1G; PP2CG; PPP2CG; MGC1675; MGC2870; PP2Cgamma; PP2C; PP2C gamma; Protein phosphatase 1C; Protein phosphatase 2C gamma isoform; Protein phosphatase 2 catalytic subunit gamma isoform; Protein phosphatase magnesium dependent 1 gamma.; anti-PPM1G antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
k1G6
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
546
Applicable Applications for anti-PPM1G antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
Antigen Species
Human
Immunogen
Recombinant human PPM1G (317-546aa) purified from E. coli
Clone Note
Anti-human PPM1G mAb, clone k1G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPM1G protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The 293T cell lysate (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The 293T cell lysate (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunohistochemistry (IHC)
(Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunohistochemistry (IHC)
(Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunofluorescence (IF)
(ICC/IF analysis of PPM1G in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
Immunofluorescence (IF)
(ICC/IF analysis of PPM1G in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
Related Product Information for anti-PPM1G antibody
PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.
Product Categories/Family for anti-PPM1G antibody
References
Suh EJ., et al. (2006) FEBS Lett 580 (26):6100-6104; Marc Flajolet., et al. (2003) Proc Natl Acad Sci 100 (26):16006-16011
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,272 Da
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1G
NCBI Official Symbol
PPM1G
NCBI Official Synonym Symbols
PP2CG; PPP2CG; PP2CGAMMA
NCBI Protein Information
protein phosphatase 1G
UniProt Protein Name
Protein phosphatase 1G
Protein Family
UniProt Gene Name
PPM1G
UniProt Synonym Gene Names
PPM1C; PP2C-gamma
NCBI Description
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
Research Articles on PPM1G
Similar Products
Product Notes
The PPM1G ppm1g (Catalog #AAA200103) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPM1G antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1G can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50. Researchers should empirically determine the suitability of the PPM1G ppm1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
