Glycogen Phosphorylase, Muscle (PYGM) Recombinant Protein | PYGM recombinant protein

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

Cat. Num. AAA2011599

• Gene Names: Pygm; PG; AI115133
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
• Purity: >98%
• Synonyms: Glycogen Phosphorylase; Muscle (PYGM); Recombinant Glycogen Phosphorylase; PYGM recombinant protein

• Host: E Coli
• Purity/Purification: >98%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RKQISVRGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYG
• Sequence Length: 842

• Applicable Applications for PYGM recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg11~Gly187 with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted isoelectric point: 6.0
• Predicted Molecular Mass: 21.7kDa
• Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Tissue Specificity: Skeletal Muscle, Brain, Heart, Kidney.
• Traits: Freeze-dried powder
• Note: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affect the charge of the protein.; 3. Post-translational modification:Phosphorylation, glycosylation, methylation etc.; 4. Post-translation clevage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 6755256
• NCBI GeneID: 19309
• NCBI Accession #: NP_035354.1
• NCBI GenBank Nucleotide #: NM_011224.1
• UniProt Accession #: Q9WUB3
• Molecular Weight: Predicted Molecular Weight: 22.0kDa; Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: glycogen phosphorylase, muscle form
• NCBI Official Synonym Full Names: muscle glycogen phosphorylase
• NCBI Official Symbol: Pygm
• NCBI Official Synonym Symbols: PG; AI115133
• NCBI Protein Information: glycogen phosphorylase, muscle form; myophosphorylase
• UniProt Protein Name: Glycogen phosphorylase, muscle form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: Pygm
• UniProt Entry Name: PYGM_MOUSE

Uniprot Description

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Phosphorylase; Transferase; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose; Endoplasmic reticulum

Cellular Component: sarcoplasmic reticulum; cytoplasm; Z disc

Molecular Function: transferase activity; glycogen phosphorylase activity; transferase activity, transferring glycosyl groups; phosphorylase activity; nucleotide binding; drug binding; carbohydrate binding; catalytic activity; pyridoxal phosphate binding; AMP binding

Biological Process: cellular calcium ion homeostasis; glycogen metabolic process; response to organic substance; glycogen catabolic process; response to cAMP; metabolic process; carbohydrate metabolic process; response to hypoxia