Sequence Information
Neuropilin 2 (NRP2) Recombinant Protein | NRP2 recombinant protein
Recombinant Neuropilin 2 (NRP2)
Reactivity
Rat
Applications
SDS-Page, Western Blot
Purity
>98%
Synonyms
Neuropilin 2 (NRP2); Recombinant Neuropilin 2 (NRP2); NRP2 recombinant protein
Host
E. coli
Reactivity
Rat
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Concentration
200ug/mL (varies by lot)
Applicable Applications for NRP2 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Glu652~Pro858
Tags
Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity
Brain, Liver.
Subcellular Location
Membrane; Single-pass type I membrane protein
Traits
Freeze-dried powder
Predicted isoelectric point
5.3
Predicted Molecular Mass
53.3kDa
Accurate Molecular Mass
58kDa as determined by SDS-PAGE reducing conditions
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neuropilin-2
NCBI Official Synonym Full Names
neuropilin 2
NCBI Official Symbol
Nrp2
NCBI Protein Information
neuropilin-2; vascular endothelial cell growth factor 165 receptor 2
UniProt Protein Name
Neuropilin-2
Protein Family
UniProt Gene Name
Nrp2
UniProt Entry Name
NRP2_RAT
NCBI Description
an axonal guidance molecule [RGD, Feb 2006]
Uniprot Description
Function: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform ofPGF.
Subunit structure: Heterodimer with NRP1. Binds PLXNB1
By similarity.
Subcellular location: Membrane; Single-pass type I membrane protein.
Tissue specificity: Found in certain neuronal populations of the CNS and in other non-neuronal tissues including mesenchymal tissue lining in the ribs.
Domain: The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding
By similarity.
Sequence similarities: Belongs to the neuropilin family.Contains 2 CUB domains.Contains 2 F5/8 type C domains.Contains 1 MAM domain.
Research Articles on NRP2
Similar Products
Product Notes
The NRP2 nrp2 (Catalog #AAA2011819) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Neuropilin 2 (NRP2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuropilin 2 (NRP2) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the NRP2 nrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuropilin 2 (NRP2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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