Sequence Information
Inter Alpha-Globulin Inhibitor H5 (ITIH5) Recombinant Protein | ITIH5 recombinant protein
Recombinant Inter Alpha-Globulin Inhibitor H5 (ITIH5)
Gene Names
Itih5; E130106B02; 4631408O11Rik; 5430408M01Rik
Applications
SDS-Page, Western Blot
Purity
>90%
Synonyms
Inter Alpha-Globulin Inhibitor H5 (ITIH5); Recombinant Inter Alpha-Globulin Inhibitor H5 (ITIH5); ITIH5 recombinant protein
Host
E. coli
Purity/Purification
>90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200 ug/mL (varies by lot)
Applicable Applications for ITIH5 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression.
Residues
Asp569-Gln737
Tags
N-terminal His-Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.8
Predicted Molecular Mass
20.2kDa
Accurate Molecular Mass
25kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCI (pH8.O) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -8O°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and preCipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8°C for one month.
Aliquot and store at -8O°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and preCipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H5
NCBI Official Synonym Full Names
inter-alpha (globulin) inhibitor H5
NCBI Official Symbol
Itih5
NCBI Official Synonym Symbols
E130106B02; 4631408O11Rik; 5430408M01Rik
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H5; ITI-HC5; ITI heavy chain H5; inter-alpha-inhibitor heavy chain 5; inter-alpha trypsin inhibitor heavy chain precursor 5
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H5
Protein Family
UniProt Gene Name
Itih5
UniProt Synonym Gene Names
ITI heavy chain H5; ITI-HC5
UniProt Entry Name
ITIH5_MOUSE
Uniprot Description
ITIH5: May act as a tumor suppressor. Belongs to the ITIH family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity
Biological Process: negative regulation of peptidase activity; hyaluronan metabolic process
Research Articles on ITIH5
Similar Products
Product Notes
The ITIH5 itih5 (Catalog #AAA2011132) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Inter Alpha-Globulin Inhibitor H5 (ITIH5) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ITIH5 itih5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Inter Alpha-Globulin Inhibitor H5 (ITIH5), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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