Sequence Information
Superoxide Dismutase 3, Extracellular (SOD3) Recombinant Protein | SOD3 recombinant protein
Recombinant Superoxide Dismutase 3, Extracellular (SOD3)
Gene Names
Sod3; EC-SOD; AI314465
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
Purity
>92%
Synonyms
Superoxide Dismutase 3; Extracellular (SOD3); Recombinant Superoxide Dismutase 3; SOD3 recombinant protein
Host
E Coli
Purity/Purification
>92%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Applicable Applications for SOD3 recombinant protein
SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression.
Residues
Ser25~Thr251
Tags
N-terminal His-Tag
Tissue Specificity
Lung, Kidney, Liver.
Subcellular Location
Secreted, extracellular space.
Traits
Freeze-dried powder
Predicted isoelectric point
6.8
Predicted Molecular Mass
26.2kDa
Accurate Molecular Mass
31&33kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degreeC for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 degreeC for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
extracellular superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 3, extracellular
NCBI Official Symbol
Sod3
NCBI Official Synonym Symbols
EC-SOD; AI314465
NCBI Protein Information
extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]
UniProt Protein Name
Extracellular superoxide dismutase [Cu-Zn]
Protein Family
UniProt Gene Name
Sod3
UniProt Synonym Gene Names
EC-SOD
UniProt Entry Name
SODE_MOUSE
Uniprot Description
SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.
Protein type: EC 1.15.1.1; Secreted; Secreted, signal peptide; Oxidoreductase
Cellular Component: extracellular matrix; extracellular space; cytoplasm; extracellular region; trans-Golgi network; nucleus
Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; metal ion binding; superoxide dismutase activity; oxidoreductase activity
Biological Process: response to copper ion; removal of superoxide radicals; response to hypoxia; superoxide metabolic process
Research Articles on SOD3
Similar Products
Product Notes
The SOD3 sod3 (Catalog #AAA2010936) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Superoxide Dismutase 3, Extracellular (SOD3) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the SOD3 sod3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 3, Extracellular (SOD3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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