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Western Blot (WB) (Western Blot; Sample: Recombinant APOA4, Mouse.)

Rabbit anti-Mouse Apolipoprotein A4 (APOA4) Polyclonal Antibody | anti-APOA4 antibody

Polyclonal Antibody to Apolipoprotein A4 (APOA4)

Gene Names
Apoa4; Apoa-4
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Apolipoprotein A4 (APOA4); Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein A4 (APOA4); anti-APOA4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.93mg/ml (varies by lot)
Applicable Applications for anti-APOA4 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemsitry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
I mmunohistochemistry: 5-20ug/mL
I mmunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant APOA4 (Gln299~Ala369) expressed in E.coli (MBS2009973)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant APOA4, Mouse.)

Western Blot (WB) (Western Blot; Sample: Recombinant APOA4, Mouse.)

Western Blot (WB)

(Western Blot; Sample: Mouse Lung lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse APOA4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Western Blot (WB) (Western Blot; Sample: Mouse Lung lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse APOA4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
apolipoprotein A-IV
NCBI Official Synonym Full Names
apolipoprotein A-IV
NCBI Official Symbol
Apoa4
NCBI Official Synonym Symbols
Apoa-4
NCBI Protein Information
apolipoprotein A-IV
UniProt Protein Name
Apolipoprotein A-IV
Protein Family
UniProt Gene Name
Apoa4
UniProt Synonym Gene Names
Apo-AIV; ApoA-IV

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell adhesion; Endoplasmic reticulum; Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 A5.2|9 25.36 cM

Cellular Component: cell surface; chylomicron; extracellular matrix; extracellular region; extracellular space

Molecular Function: antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; phosphatidylcholine binding; protein homodimerization activity

Biological Process: cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neuron projection regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol transport; regulation of intestinal cholesterol absorption; removal of superoxide radicals; response to lipid hydroperoxide; reverse cholesterol transport; transport; triglyceride catabolic process

Research Articles on APOA4

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Product Notes

The APOA4 apoa4 (Catalog #AAA2002143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apolipoprotein A4 (APOA4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein A4 (APOA4) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemsitry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL I mmunohistochemistry: 5-20ug/mL I mmunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the APOA4 apoa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein A4 (APOA4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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