Apolipoprotein A4 (APOA4) Recombinant Protein | APOA4 recombinant protein

Recombinant Apolipoprotein A4 (APOA4)

Cat. Num. AAA2009973

• Gene Names: Apoa4; Apoa-4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apolipoprotein A4 (APOA4); Recombinant Apolipoprotein A4 (APOA4); APOA4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-QV EEFRRTVEPM GEMFNKALVQ QLEQFRQQLG PNSGEVESHL SFLEKSLREK VNSFMSTLEK KGSPDQPQA
• Sequence Length: 395

• Applicable Applications for APOA4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln299~Ala369 (Accession # P06728) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110347473
• NCBI GeneID: 11808
• NCBI Accession #: NP_031494.2
• NCBI GenBank Nucleotide #: NM_007468.2
• UniProt Accession #: P06728; Q91XF8
• Molecular Weight: 13.9kDa
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: Apoa4
• NCBI Official Synonym Symbols: Apoa-4
• NCBI Protein Information: apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_MOUSE

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Lipid-binding; Endoplasmic reticulum; Cell adhesion; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; chylomicron; cell surface; extracellular region

Molecular Function: antioxidant activity; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: multicellular organismal lipid catabolic process; positive regulation of fatty acid biosynthetic process; cholesterol metabolic process; removal of superoxide radicals; neurite regeneration; protein-lipid complex assembly; lipid homeostasis; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; regulation of cholesterol absorption; innate immune response in mucosa; response to lipid hydroperoxide; transport; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport