Mouse apolipoprotein A-IV ELISA Kit | APOA4 elisa kit

Mouse Apolipoprotein A-IV, APOA4 ELISA Kit

Cat. Num. AAA915059

• Gene Names: Apoa4; Apoa-4
• Reactivity: Mouse
• Synonyms: apolipoprotein A-IV; Mouse Apolipoprotein A-IV; APOA4 ELISA Kit; Mouse Apolipoprotein A-IV (APOA4) ELISA kit; MGC142154; MGC142156; ; APOA4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse APOA4. No significant cross-reactivity or interference between mouse APOA4 and analogues was observed.
• Sequence Length: 395

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 12.5 ng/ml -800 ng/ml.
• Sensitivity: The minimum detectable dose of mouse APOA4 is typically less than 3.12 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA4 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110347473
• NCBI GeneID: 11808
• NCBI Accession #: NP_031494.2
• NCBI GenBank Nucleotide #: NM_007468.2
• UniProt Accession #: P06728; Q91XF8
• Molecular Weight: 45,029 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: Apoa4
• NCBI Official Synonym Symbols: Apoa-4
• NCBI Protein Information: apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_MOUSE

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell adhesion; Lipid-binding; Secreted, signal peptide; Endoplasmic reticulum; Secreted

Cellular Component: extracellular space; chylomicron; cell surface; extracellular region

Molecular Function: antioxidant activity; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: multicellular organismal lipid catabolic process; cholesterol metabolic process; positive regulation of fatty acid biosynthetic process; removal of superoxide radicals; protein-lipid complex assembly; neurite regeneration; lipid homeostasis; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; phospholipid efflux; regulation of cholesterol absorption; transport; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport