Mouse Apolipoprotein A-IV ELISA Kit | Apoa4 elisa kit

Mouse Apolipoprotein A-IV ELISA Kit

Cat. Num. AAA2882319

• Gene Names: Apoa4; Apoa-4
• Reactivity: Mouse
• Synonyms: Apolipoprotein A-IV; Mouse Apolipoprotein A-IV ELISA Kit; Apo-AIV; ApoA-IV; Apolipoprotein A4; Apoa4; Apoa4 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Apolipoprotein A-IV,Apo-AIV
• Sequence Length: 395

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: < 0.403 ng/mL
• Intra-assay Precision: <=5.4%
• Inter-assay Precision: <=7.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Apoa4 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110347473
• NCBI GeneID: 11808
• NCBI Accession #: NP_031494.2
• NCBI GenBank Nucleotide #: NM_007468.2
• UniProt Accession #: P06728; Q91XF8
• Molecular Weight: 45,029 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: Apoa4
• NCBI Official Synonym Symbols: Apoa-4
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_MOUSE

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Endoplasmic reticulum; Lipid-binding

Cellular Component: cell surface; chylomicron; extracellular region; extracellular space

Molecular Function: antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; phosphatidylcholine binding; protein homodimerization activity

Biological Process: cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol absorption; regulation of cholesterol transport; removal of superoxide radicals; response to lipid hydroperoxide; reverse cholesterol transport; transport; triacylglycerol catabolic process