Human WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit | WWP2 elisa kit

Human WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit

Cat. Num. AAA2000038

• Gene Names: WWP2; AIP2; WWp2-like
• Reactivity: Human
• Synonyms: WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2); Human WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit; AIP2; WWp2-like; Atrophin-1-interacting protein 2; NEDD4-like E3 ubiquitin-protein ligase WWP2; WWP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2).; No significant cross-reactivity or interference between WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) and analogues was observed.
• Sequence Length: 870

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WWP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for WWP2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 15489060
• NCBI GeneID: 11060
• UniProt Accession #: O00308; Q6ZTQ5; Q96CZ2; Q9BWN6; A6NEP1; B2R706; B4DTL5; F5H213; H3BRF3; I3RSG8
• Molecular Weight: 35,248 Da
• NCBI Official Full Name: WW domain containing E3 ubiquitin protein ligase 2
• NCBI Official Synonym Full Names: WW domain containing E3 ubiquitin protein ligase 2
• NCBI Official Symbol: WWP2
• NCBI Official Synonym Symbols: AIP2; WWp2-like
• NCBI Protein Information: NEDD4-like E3 ubiquitin-protein ligase WWP2
• UniProt Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• Protein Family: NEDD4-like E3 ubiquitin-protein ligase
• UniProt Gene Name: WWP2
• UniProt Synonym Gene Names: AIP2
• UniProt Entry Name: WWP2_HUMAN

NCBI Description

This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]

Uniprot Description

WWP2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation- induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

Protein type: Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; transcription factor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; entry of virus into host cell; protein autoubiquitination; regulation of membrane potential; negative regulation of transcription factor activity; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; protein modification process; negative regulation of protein transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transporter activity