Rat anti-Mouse CD272 Monoclonal Antibody | anti-CD272 antibody

Anti-Mouse CD272 Monoclonal Antibody (APC Conjugated) [PK18.6]

Cat. Num. AAA2558185

• Gene Names: Btla; A630002H24
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD272; Monoclonal Antibody; Anti-Mouse CD272 Monoclonal Antibody (APC Conjugated) [PK18.6]; B- and T-lymphocyte attenuator; Btla; B- and T-lymphocyte-associated protein; anti-CD272 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: PK18.6
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 306

• Applicable Applications for anti-CD272 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: APC
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD272 Monoclonal Antibody(APC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD272 antibody

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 83700227
• NCBI GeneID: 208154
• NCBI Accession #: NP_001032808.2
• NCBI GenBank Nucleotide #: NP_001032808.2
• UniProt Accession #: Q7TSA3
• NCBI Official Full Name: B- and T-lymphocyte attenuator isoform 1
• NCBI Official Synonym Full Names: B and T lymphocyte associated
• NCBI Official Symbol: Btla
• NCBI Official Synonym Symbols: A630002H24
• NCBI Protein Information: B- and T-lymphocyte attenuator
• UniProt Protein Name: B- and T-lymphocyte attenuator
• UniProt Gene Name: Btla
• UniProt Entry Name: BTLA_MOUSE

Uniprot Description

BTLA: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: cell surface receptor linked signal transduction; negative regulation of T cell proliferation; immune system process; negative regulation of alpha-beta T cell proliferation; immune response-regulating cell surface receptor signaling pathway; negative regulation of B cell proliferation