Human Filamin A Alpha (FLNa) ELISA Kit | FLNa elisa kit

Human Filamin A Alpha (FLNa) ELISA Kit

Cat. Num. AAA2704286

• Gene Names: FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• Reactivity: Human
• Synonyms: Filamin A Alpha (FLNa); Human Filamin A Alpha (FLNa) ELISA Kit; ABP280; FLN-A; ABPX; FLN; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; Actin Binding Protein 280; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin; FLNa elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Filamin A Alpha (FLNa).; No significant cross-reactivity or interference between Filamin A Alpha (FLNa) and analogues was observed.
• Sequence Length: 2,639

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 1.88-120ng/mL
• Sensitivity: < 0.65ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin A Alpha (FLNa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin A Alpha (FLNa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLNa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha (FLNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin A Alpha (FLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha (FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha (FLNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119593152
• NCBI GeneID: 2316
• UniProt Accession #: P21333; Q5HY53; Q5HY55; Q8NF52; E9KL45
• Molecular Weight: 280,018 Da
• NCBI Official Full Name: filamin A, alpha (actin binding protein 280), isoform CRA_c
• NCBI Official Synonym Full Names: filamin A
• NCBI Official Symbol: FLNA
• NCBI Official Synonym Symbols: FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• NCBI Protein Information: filamin-A
• UniProt Protein Name: Filamin-A
• Protein Family: Filamin
• UniProt Gene Name: FLNA
• UniProt Synonym Gene Names: FLN; FLN1; FLN-A; ABP-280

NCBI Description

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance ().