WWP2 recombinant protein

WWP2 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121280

• Gene Names: WWP2; AIP2; WWp2-like
• Purity: >90% as determined by SDS-PAGE
• Synonyms: WWP2; WWP2 Protein; Human; Recombinant (His & GST Tag); Human WWP2 Protein (His & GST Tag); AIP2 Protein; WWp2-like Protein; WW domain containing E3 ubiquitin protein ligase 2; WWP2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu870

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human WWP2 (O00308)(Met1-Glu870) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for WWP2 recombinant protein

Background: WWP2 contains 1 C2 domain, 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain and 4 WW domains. It is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. WWP2 can be detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. It is also expressed in spleen and peripheral blood leukocytes. WWP2 polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. WWP2 ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. It also ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. WWP2 ubiquitinates RPB1 and promotes it to proteasomal degradation.

References

McDonald FJ, et al. (2002) Ubiquitin-protein ligase WWP2 binds to and downregulates the epithelial Na(+) channel. Am J Physiol Renal Physiol. 283 (3): F431-6.Soond SM, et al. (2011) Selective targeting of activating and inhibitory Smads by distinct WWP2 ubiquitin ligase isoforms differentially modulates TGFbeta signalling and EMT. Oncogene. 30 (21): 2451-62.Marcucci R, et al. (2011) Pin1 and WWP2 regulate GluR2 Q/R site RNA editing by ADAR2 with opposing effects. EMBO J. 30 (20): 4211-22.Maddika S, et al. (2011) WWP2 is an E3 ubiquitin ligase for PTEN. Nat Cell Biol. 13 (6): 728-33.Xu H, et al. (2009) WWP2 promotes degradation of transcription factor OCT4 in human embryonic stem cells. Cell Res. 19 (5): 561-73.

NCBI and Uniprot Product Information

• NCBI GI #: 32171765
• NCBI GeneID: 11060
• UniProt Accession #: O00308; Q6ZTQ5; Q96CZ2; Q9BWN6; A6NEP1; B2R706; B4DTL5; F5H213; H3BRF3; I3RSG8
• Molecular Weight: Observed MW 105kDa; Calculated MW 35kDa/51kDa/86kDa/98kDa
• NCBI Official Full Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• NCBI Official Synonym Full Names: WW domain containing E3 ubiquitin protein ligase 2
• NCBI Official Symbol: WWP2
• NCBI Official Synonym Symbols: AIP2; WWp2-like
• NCBI Protein Information: NEDD4-like E3 ubiquitin-protein ligase WWP2; atrophin-1 interacting protein 2
• UniProt Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• Protein Family: NEDD4-like E3 ubiquitin-protein ligase
• UniProt Gene Name: WWP2
• UniProt Synonym Gene Names: AIP2
• UniProt Entry Name: WWP2_HUMAN

NCBI Description

This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]

Uniprot Description

WWP2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation- induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; transcription factor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; entry of virus into host cell; regulation of membrane potential; protein autoubiquitination; negative regulation of transcription factor activity; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; protein modification process; negative regulation of protein transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transporter activity