Mouse anti-Human VAMP-1,2/Synaptobrevin Monoclonal Antibody | anti-VAMP1 antibody

Anti-VAMP-1,2/Synaptobrevin Monoclonal Antibody

Cat. Num. AAA190554

• Gene Names: VAMP1; SYB1; VAMP-1; DKFZp686H12131
• Reactivity: Human
• Applications: Immunoblot, Western Blot
• Synonyms: VAMP-1; 2/Synaptobrevin; Monoclonal Antibody; Anti-VAMP-1; 2/Synaptobrevin Monoclonal Antibody; anti-VAMP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SP-11
• Specificity: VAMP-1,2/Synaptobrevin; This antibody recognizes human VAMP1 and VAMP2.
• Form/Format: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

• Applicable Applications for anti-VAMP1 antibody: Immunoblot
• Application Notes: Immunoblotting: use at 0.5-1.0ug/ml. A band of ~18kDa is detected. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Human brain lysate
• Antigen: Crude human synaptic immunoprecipitate.
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Related Product Information for anti-VAMP1 antibody

VAMP-1 (Vesicle-Associated Membrane Protein) is an 18kDa member of the synaptobrevin family. It is expressed in neurons, neutrophils, and skeletal muscle cells and participates in vesicle fusion with the plasma membrane. Synaptobrevins, syntaxins, SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP-1 is also one of the key proteins in the SNARE complex involved in regulated exocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 40549446
• NCBI GeneID: 6843
• NCBI Accession #: NP_954740.1
• NCBI GenBank Nucleotide #: NM_199245.1
• UniProt Accession #: P23763; O75468; Q15857; Q6FG94; Q8IVC9; A8MVP3; D3DUR3
• Molecular Weight: 12,902 Da
• NCBI Official Full Name: vesicle-associated membrane protein 1 isoform 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 1 (synaptobrevin 1)
• NCBI Official Symbol: VAMP1
• NCBI Official Synonym Symbols: SYB1; VAMP-1; DKFZp686H12131
• NCBI Protein Information: vesicle-associated membrane protein 1; synaptobrevin 1; synaptobrevin-1; OTTHUMP00000238453; OTTHUMP00000238454; OTTHUMP00000238455; OTTHUMP00000238560; OTTHUMP00000238561
• UniProt Protein Name: Vesicle-associated membrane protein 1
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP1
• UniProt Synonym Gene Names: SYB1
• UniProt Entry Name: VAMP1_HUMAN

NCBI Description

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Uniprot Description

VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p

Cellular Component: SNARE complex; mitochondrial outer membrane; synaptic vesicle; neuron projection; cell surface; synaptic vesicle membrane; integral to plasma membrane; cytosol; cell junction

Molecular Function: SNAP receptor activity; SNARE binding; protein binding

Biological Process: vesicle fusion; exocytosis; pathogenesis

Disease: Spastic Ataxia 1, Autosomal Dominant