Mouse HLA Class I Antibody
Anti-HLA Class I Purified Low Endotoxin
Reactivity
Cow, Non-human primates, Human, Cat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
HLA Class I; Anti-HLA Class I Purified Low Endotoxin; anti-HLA Class I antibody
Host
Mouse
Reactivity
Cow, Non-human primates, Human, Cat
Isotype
IgG2a
Clone Number
W6/32
Specificity
The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-HLA Class I antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), ELISA (EIA), MC (CyTOF), Functional
Application Notes
FC/FACS: QC tested
Immunogen
Membrane of human tonsil cells
Negative Species
Rabbit
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-HLA Class I antibody
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
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Product Notes
The HLA Class I (Catalog #AAA1801702) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA Class I Purified Low Endotoxin reacts with Cow, Non-human primates, Human, Cat and may cross-react with other species as described in the data sheet. AAA Biotech's HLA Class I can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), ELISA (EIA), MC (CyTOF), Functional. FC/FACS: QC tested. Researchers should empirically determine the suitability of the HLA Class I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA Class I, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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