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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-HLA-A2 (BB7.2) Alexa Fluor 647.)

Mouse anti-Human HLA-A2 Monoclonal Antibody | anti-HLA-A2 antibody

Anti-HLA-A2 Alexa Fluor647

Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
HLA-A2; Monoclonal Antibody; Anti-HLA-A2 Alexa Fluor647; anti-HLA-A2 antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
BB7.2
Specificity
The antibody BB7.2 recognizes an extracellular epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen.
Purity/Purification
The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Applicable Applications for anti-HLA-A2 antibody
Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 5ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.5 ml) is sufficient for 100 tests.
Conjugation
Alexa Fluor647
Immunogen
HLA-A2 solubilised by papain
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-HLA-A2 (BB7.2) Alexa Fluor 647.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-HLA-A2 (BB7.2) Alexa Fluor 647.)
Related Product Information for anti-HLA-A2 antibody
HLA-A2 (44kDa) is the most frequent HLA-A allele in human ethnic populations. HLA-A, together with HLA-B and HLA-C, represent human HLA class I major histocompatibility (MHC) antigens. These intrinsic membrane glycoproteins are expressed on nucleated cells and noncovalently associate with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence.
References
*Lee L, Loftus D, Appella E, Margulies DH, Mage M: A recombinant single-chain HLA-A2.1 molecule, with a cis active beta-2-microglobulin domain, is biologically active in peptide binding and antigen presentation. Hum Immunol. 1996 Aug;49(1):28-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/8839773ordina,*Forero L, Zwirner NW, Fink CW, Fernández-Viña MA, Stastny P: Juvenile arthritis, HLA-A2 and binding of DEK oncogene-peptides. Hum Immunol. 1998 Jul;59(7):443-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/9684994ordina,*Verdegaal EM, Huinink DB, Hoogstraten C, Marijnissen AK, Gorsira MB, Claas FH, Osanto S: Isolation of broadly reactive, tumor-specific, HLA Class-I restricted CTL from blood lymphocytes of a breast cancer patient. Hum Immunol. 1999 Dec;60(12):1195-206., URL: https://www.ncbi.nlm.nih.gov/pubmed/10626733ordin,*Santin AD, Bellone S, Ravaggi A, Pecorelli S, Cannon MJ, Parham GP: Induction of ovarian tumor-specific CD8+ cytotoxic T lymphocytes by acid-eluted peptide-pulsed autologous dendritic cells. Obstet Gynecol. 2000 Sep;96(3):422-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/10960637ordin,*Liang B, Zhu L, Liang Z, Weng X, Lu X, Zhang C, Li H, Wu X: A simplified PCR-SSP method for HLA-A2 subtype in a population of Wuhan, China. Cell Mol Immunol. 2006 Dec;3(6):453-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17257499ordin,*Santos-Aguado J, Barbosa JA, Biro PA, Strominger JL: Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J Immunol. 1988 Oct 15;141(8):2811-8.", URL: https://www.ncbi.nlm.nih.gov/pubmed/2459229ordina,*Natali PG, Nicotra MR, Bigotti A, Venturo I, Marcenaro L, Giacomini P, Russo C: Selective changes in expression of HLA class I polymorphic determinants in human solid tumors. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6719-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/2672003ordina,*Hogan KT, Brown SL: Localization and characterization of serologic epitopes on HLA-A2. Hum Immunol. 1992 Mar;33(3):185-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/1377666ordina,*Nijman HW, Houbiers JG, Vierboom MP, van der Burg SH, Drijfhout JW, D'Amaro J, Kenemans P, Melief CJ, Kast WM: Identification of peptide sequences that potentially trigger HLA-A2.1-restricted cytotoxic T lymphocytes. Eur J Immunol. 1993 Jun;23(6):1215-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/7684681ordina,*Peoples GE, Goedegebuure PS, Andrews JV, Schoof DD, Eberlein TJ: HLA-A2 presents shared tumor-associated antigens derived from endogenous proteins in ovarian cancer. J Immunol. 1993 Nov 15;151(10):5481-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/8228240ordina,*Barouch D, Davenport M, McMichael A, Reay P: A mAb against HLA-A2 can be influenced both positively and negatively by the associated peptide. Int Immunol. 1995 Oct;7(10):1599-605., URL: https://www.ncbi.nlm.nih.gov/pubmed/8562505ordina,*Scornik JC: HLA-A2 epitopes recognized by alloantibodies from broadly sensitized patients. Hum Immunol. 1987 Apr;18(4):277-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/2437089ordina

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Product Notes

The HLA-A2 (Catalog #AAA1802031) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA-A2 Alexa Fluor647 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-A2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 5ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.5 ml) is sufficient for 100 tests. Researchers should empirically determine the suitability of the HLA-A2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-A2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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