Mouse anti-Human HLA-ABCE Monoclonal Antibody | anti-HLA-ABCE antibody

Anti-HLA-ABCE Alexa Fluor488

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: HLA-ABCE; Monoclonal Antibody; Anti-HLA-ABCE Alexa Fluor488; anti-HLA-ABCE antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: TP25.99SF
• Specificity: The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-HLA-ABCE antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB)
• Application Notes: FC/FACS: Recommended dilution: 1-5ug/ml
• Conjugation: Alexa Fluor488
• Immunogen: IFN-gamma-treated human melanoma cells Colo 38
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-ABCE (TP25.99SF) Alexa Fluor 488.)

Related Product Information for anti-HLA-ABCE antibody

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

References

*Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and Functional Application (FUNC)tional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148(10):3202-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=tanabe+m,*Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H, Moy FJ, Powers R, Ferrone S: Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta 2-microglobulin-associated and beta 2-microglobulin-free HLA class I heavy chains. J Immunol. 2000 Sep 15;165(6):3275-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/10975844,*Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F: Beta 2-microglobulin-free HLA class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol. 2003 Aug 15;171(4):1918-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12902494,*Moy FJ, Desai SA, Wang X, Noronha EJ, Zhou Q, Ferrone S, Powers R: Analysis by NMR spectroscopy of the structural homology between the linear and the cyclic peptide recognized by anti-human leukocyte antigen class I monoclonal antibody TP25.99. J Biol Chem. 2000 Aug 11;275(32):24679-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/10825172,*Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61(11):1074-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=blaschit,*Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=paul+p+r