Mouse anti-Human HLA Class I Antibody | anti-HLA-C antibody

Anti-HLA Class I Purified

Cat. Num. AAA1801031

• Gene Names: HLA-C; MHC; HLAC; HLC-C; D6S204; PSORS1; HLA-JY3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: HLA Class I; Anti-HLA Class I Purified; anti-HLA-C antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: MEM-147
• Specificity: The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 298

• Applicable Applications for anti-HLA-C antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: FC/FACS: QC tested
• Immunogen: PHA-activated peripheral blood lymphocytes
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.)

Related Product Information for anti-HLA-C antibody

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

NCBI and Uniprot Product Information

• NCBI GI #: 817046176
• NCBI GeneID: 3107
• NCBI Official Full Name: HLA class I, partial
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, C
• NCBI Official Symbol: HLA-C
• NCBI Official Synonym Symbols: MHC; HLAC; HLC-C; D6S204; PSORS1; HLA-JY3
• NCBI Protein Information: HLA class I histocompatibility antigen, C alpha chain
• Protein Family: HLA class I histocompatibility antigen

NCBI Description

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]