Mouse Epiregulin PicoKine ELISA Kit | Ereg elisa kit

Mouse Epiregulin PicoKine ELISA Kit

Cat. Num. AAA177602

• Gene Names: Ereg; EPR
• Reactivity: Mouse
• Synonyms: Epiregulin PicoKine; Mouse Epiregulin PicoKine ELISA Kit; Proepiregulin; Epiregulin; EPR; ER; Ereg; EREG_HUMAN; Proepiregulin ; epiregulin; Ereg elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Epiregulin
• Sequence Length: 162

• Assay Range: 125pg/ml-8000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse Epiregulin in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, V53-L101
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml mouse Epiregulin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Epiregulin standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1257|3204|5394|1020|3562|4975; Standard Deviation|82.96|189.03|350.61|73.44|23.865|368.15; CV(%)|6.6|5.9|6.5|7.2|6.7|7.4
• Sample Data: Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000; O.D.|0.009|0.049|0.088|0.201|0.374|0.832|1.402|2.053
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse Epiregulin PicoKine ELISA Kit standard curve )

Related Product Information for Ereg elisa kit

Principle of the assay: Mouse Epiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Epiregulin has been precoated onto 96-well plates. Standards (E Coli, V53-L101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Epiregulin amount of sample captured in plate.

Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 6679683
• NCBI GeneID: 13874
• NCBI Accession #: NP_031976.1
• NCBI GenBank Nucleotide #: NM_007950.2
• UniProt Accession #: Q61521; Q7M0A8
• Molecular Weight: 18,474 Da
• NCBI Official Full Name: proepiregulin preproprotein
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: Ereg
• NCBI Official Synonym Symbols: EPR
• NCBI Protein Information: proepiregulin
• UniProt Protein Name: Proepiregulin
• Protein Family: Proepiregulin
• UniProt Gene Name: Ereg
• UniProt Synonym Gene Names: EPR
• UniProt Entry Name: EREG_MOUSE

Uniprot Description

Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

Protein type: Membrane protein, integral

Cellular Component: extracellular region; extracellular space; integral to membrane; membrane; plasma membrane

Molecular Function: epidermal growth factor receptor binding; growth factor activity

Biological Process: anatomical structure morphogenesis; angiogenesis; cell differentiation; cell-cell signaling; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; female meiosis; keratinocyte proliferation; luteinizing hormone signaling pathway; mRNA transcription; multicellular organismal development; negative regulation of cell proliferation; negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; oocyte maturation; organ morphogenesis; ovarian cumulus expansion; ovulation; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of cytokine biosynthetic process; positive regulation of cytokine production; positive regulation of DNA replication; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of innate immune response; positive regulation of interleukin-6 biosynthetic process; positive regulation of mitosis; positive regulation of phosphorylation; positive regulation of protein kinase activity; positive regulation of smooth muscle cell proliferation; primary follicle stage, oogenesis