Mouse Epiregulin ELISA Kit | EREG elisa kit

Mouse Epiregulin ELISA Kit

Cat. Num. AAA8248433

• Gene Names: Ereg; EPR
• Reactivity: Mouse
• Synonyms: Epiregulin; Mouse Epiregulin ELISA Kit; Proepiregulin; EREG elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Epiregulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Epiregulin proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 162

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EREG elisa kit

Principle of the Assay: The Mouse Epiregulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse Epiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 6679683
• NCBI GeneID: 13874
• NCBI Accession #: NP_031976.1
• NCBI GenBank Nucleotide #: NM_007950.2
• UniProt Accession #: Q61521; Q7M0A8
• Molecular Weight: 18,474 Da
• NCBI Official Full Name: proepiregulin preproprotein
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: Ereg
• NCBI Official Synonym Symbols: EPR
• NCBI Protein Information: proepiregulin
• UniProt Protein Name: Proepiregulin
• Protein Family: Proepiregulin
• UniProt Gene Name: Ereg
• UniProt Synonym Gene Names: EPR
• UniProt Entry Name: EREG_MOUSE

Uniprot Description

Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

Protein type: Membrane protein, integral

Cellular Component: extracellular space

Molecular Function: epidermal growth factor receptor binding; growth factor activity

Biological Process: anatomical structure morphogenesis; cell-cell signaling; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; female meiosis; keratinocyte proliferation; luteinizing hormone signaling pathway; mRNA transcription; negative regulation of cell proliferation; negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; oocyte maturation; organ morphogenesis; ovarian cumulus expansion; ovulation; positive regulation of cell proliferation; positive regulation of cytokine biosynthetic process; positive regulation of cytokine production; positive regulation of DNA replication; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of innate immune response; positive regulation of interleukin-6 biosynthetic process; positive regulation of mitosis; positive regulation of phosphorylation; positive regulation of protein kinase activity; positive regulation of smooth muscle cell proliferation; primary follicle stage, oogenesis