Western Blot (WB)
(Anti-OSBP1 antibody, MBS176321, Western blottingLane 1: Rat Kidney Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Lung Tissue LysateLane 4: HELA Cell LysateLane 5: A549 Cell Lysate )
Rabbit OSBP1 Polyclonal Antibody | anti-OSBP antibody
Anti-OSBP1 antibody
Gene Names
OSBP; OSBP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
OSBP1; Polyclonal Antibody; Anti-OSBP1 antibody; Oxysterol-binding protein 1; oxysterol binding protein; OSBP 1 antibody; OSBP antibody; OSBP1_HUMAN antibody; Oxysterol binding protein 1 antibody; Oxysterol-binding protein 1 antibody; anti-OSBP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
807
Applicable Applications for anti-OSBP antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human OSBP1 (290-304aa WQKSLQYERDQRIRL), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-OSBP1 antibody, MBS176321, Western blottingLane 1: Rat Kidney Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Lung Tissue LysateLane 4: HELA Cell LysateLane 5: A549 Cell Lysate )
Western Blot (WB)
(Anti-OSBP1 antibody, MBS176321, Western blottingLane 1: Rat Kidney Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Lung Tissue LysateLane 4: HELA Cell LysateLane 5: A549 Cell Lysate )
Related Product Information for anti-OSBP antibody
Description: Rabbit IgG polyclonal antibody for Oxysterol-binding protein 1(OSBP) detection. Tested with WB in Human, Mouse, Rat.
Background: OSBP(Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al.(2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein(ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al.(2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al.(2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase(ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.
Background: OSBP(Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al.(2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein(ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al.(2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al.(2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase(ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
89,421 Da
NCBI Official Full Name
Oxysterol-binding protein 1
NCBI Official Synonym Full Names
oxysterol binding protein
NCBI Official Symbol
OSBP
NCBI Official Synonym Symbols
OSBP1
NCBI Protein Information
oxysterol-binding protein 1
UniProt Protein Name
Oxysterol-binding protein 1
Protein Family
UniProt Gene Name
OSBP
UniProt Synonym Gene Names
OSBP1
UniProt Entry Name
OSBP1_HUMAN
NCBI Description
Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq, Jul 2008]
Uniprot Description
OSBP: Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25- hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. Belongs to the OSBP family.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 11q12-q13
Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus; cell junction
Molecular Function: protein domain specific binding; protein binding; oxysterol binding
Biological Process: lipid transport
Research Articles on OSBP
Similar Products
Product Notes
The OSBP osbp (Catalog #AAA176321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OSBP1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OSBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the OSBP osbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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